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Acetyl-CoA Colorimetric Assay Kit

  • Cat.No.:E-BC-K652-M

  • Detection instrument: Microplate reader (330-350 nm, optimum wavelength: 340 nm)

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Price: $490

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Detection principle

Malate and NAD+ form oxoacetate and NADH under the action of malate dehydrogenase, then acetyl-coa and oxoacetate form coenzyme A and citrate under the action of citrate synthetase. The content of acetyl-coA can be calculated by measuring the change of absorbance value at 340 nm

Performance characteristics

Synonyms Acetyl-CoA
Sample type animal tissue
Sensitivity 150 nmol/mL
Detection range 150-500 nmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 15 min
Precision Average inter-assay CV: 5.300%Average intra-assay CV: 4%
Storage -20℃
Valid period 12 months



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