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Phage Display Customization Service

Phage display technology uses genetic engineering technology to display antibodies on the surface of phages. The screening of phage display antibodies is performed by their binding with the target protein. Phage libraries have the characteristics of large library capacity and high-throughput screening. Screening in natural libraries can avoid the immunization and hybridoma fusion, thus greatly shortening the antibody development time.

Elabscience® can provide customers with personalized screening strategies, build phage antibody libraries of different species according to customers' special needs, and provide comprehensive and efficient antibody sequence discovery services.

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Phage Display Customization Service

Phage Display Service Features

Immune or naïve library construction

Immune or naïve library construction

High throughput: Large library capacity from 107 to over 1012

High throughput: Large library capacity from 107 to over 1012

Antibodies development for Ag with low immunogenicity

Antibodies development for Ag with low immunogenicity

Easy to humanize

Easy to humanize

Phage Display Service List

Service Categories Steps Specification Timeline Price
Immunization Antigen preparation Small molecule, peptide, recombinant protein, native protein can be used 4 Weeks Inquiry
Immunization Host: alpaca, llama, and camel. Naïve library can also be used for screening 0-8 Weeks
Antibody Library Construction cDNA synthesis B cell sorting, RNA extraction, and RT-PCR to get cDNA 1 Week
Phagemid PCR to amplify antibody gene and insert into phage vector 4 Weeks
Library construction and identification Identify the diversity and capacity of the library to ensure that the library capacity is greater than 108 and the correct insertion rate is greater than 90% 2 Weeks
Antibody library Phage display antibody library is obtained by phage rescue 1 Week
Enriching and Screening After 3-4 rounds of panning, antibodies with an affinity of more than 10-7M were obtained 3-4 Weeks
Delivery 3-5 antibody sequences will be given according to standard protocol. Three positive clones will be delivered If you need more -
Related Service Antibody expression According to customer requirements, scFv, Fab, and VHH can be expressed by prokaryotic expression system; intact IgG molecules can be expressed by CHO / 293 cells
Affinity assay -
Epitope identification -

Service Process

Antigen preparation

Antigen preparation

Immunization

Immunization

Library Construction

Library construction

Biopanning and Library screening

Biopanning and library screening

Antibody sequencing and expression

Antibody sequencing and expression

SPR analysis

SPR analysis

Projects Accomplished

The nanoantibody sequence obtained through phage display technology is recombined and expressed, labeled with biotin, and accurately displayed as a tumor cell surface marker protein for prostate cancer through an immunofluorescence detection method.

Projects Accomplished