Fluorochromes
Elabscience® offers a wide range of fluorochrome dyes that span different spectra and are suitable for various detection channels. Additionally, activated dyes are available to fulfill your more specific experimental requirements.
In selecting dyes, it is essential to choose based on the key characteristics of the dyes, such as the relative brightness of the fluorochrome dye, its wavelength range, potential spillover with other fluorochromes, and considering the instrument's laser and detection channel configurations. When performing multi-color detection, the spillover between different dyes within the same channel must be taken into account. Elabscience® provides the necessary information on various fluorochrome dyes to aid in your selection.
Fluorochromes Staining Results (Partial)
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APC Anti-Mouse Foxp3 (E-AB-F1238E)
C57BL/6 splenocytes are surface stained with FITC Anti-Mouse CD4 Antibody[GK1.5] followed by fixation and permeabilization using the Foxp3/Transcription Factor Staining Kit and intracellular staining with APC Anti-Mouse Foxp3 Monoclonal Antibody.
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PE Anti-Human CD56 (E-AB-F1239D)
Human peripheral blood lymphocytes are stained with APC Anti-Human CD3 Antibody and PE Anti-Human CD56 Antibody (Left). Lymphocytes are stained with APC Anti-Human CD3 Antibody and PE Mouse IgG1, κ Isotype Control (Right).
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PerCP Anti-Human CD8a (E-AB-F1110F)
Human peripheral blood PBMC cells are stained with PerCP Anti-Human CD8a Antibody.
APC Anti-Mouse Foxp3 (E-AB-F1238E)
C57BL/6 splenocytes are surface stained with FITC Anti-Mouse CD4 Antibody[GK1.5] followed by fixation and permeabilization using the Foxp3/Transcription Factor Staining Kit and intracellular staining with APC Anti-Mouse Foxp3 Monoclonal Antibody.
PE Anti-Human CD56 (E-AB-F1239D)
Human peripheral blood lymphocytes are stained with APC Anti-Human CD3 Antibody and PE Anti-Human CD56 Antibody (Left). Lymphocytes are stained with APC Anti-Human CD3 Antibody and PE Mouse IgG1, κ Isotype Control (Right).
PerCP Anti-Human CD8a (E-AB-F1110F)
Human peripheral blood PBMC cells are stained with PerCP Anti-Human CD8a Antibody.
Elabscience® Hot Fluorochrome Dyes
| Product Name | Color | Excitation Laser Lines (nm) | Excitation Max (nm) | Emission Max (nm) |
|---|---|---|---|---|
| PE (R-Phycoerythrin) | Yellow | 488/561 | 495, 565 | 575 |
| APC (Allophycocyanin) | Red | 633 | 650 | 660 |
| PerCP (Peridinin-Chlorophyll-Protein Complex) | Red | 488 | 440, 480, 675 | 675 |
| Cross-linked APC | Red | 633 | 650 | 660 |
| SMCC Activated R-PE | Yellow | 488/561 | 495, 565 | 575 |
| SMCC Activated APC | Red | 633 | 650 | 660 |
| SMCC Activated PerCP | Red | 488 | 440, 480, 675 | 675 |
| SMCC Activated Peroxidase (HRP) | — | — | — | — |
