Panel Design Services
Elabscience provides professional and free Panel Design Services to customers. You only need to provide your flow cytometry experiment marker information (specificities, logical relationships or references, expression levels of target markers) and basic flow cytometer information (laser, detection channel, and filter information) to our technical support. We will provide professional and free Panel Design Services for your experiment. Please fill out the Panel Design Service Request Table.
Features of Panel Design Services
• Personalization: Customized according to your experiment to ensure that the panel design meets your experimental requirements and instrument configuration.
• Professionalism: Experienced flow cytometry technical support provides you with high-quality panel design solutions.
• Convenience: Saves you time and energy, allowing you to focus more on experimental operations and related work.
Process of Panel Design Services
- Basic Information Provided
- Select the Target Markers
- Check the Flow Cytometry Information
- Check Fluorochrome Information
- Pair Antigens with Fluorochromes
- Provide the Panel Design
Elabscience Techsupport Design The Panel
Click to download the Panel Design Service Request Table.
The full service process takes 1-2 business days. For more detailed information, please contact us for consultation.
Elabscience also provides professional and free Flow Cytometry Data Analysis Service, with one-stop flow cytometry solution.
To learn about the common immune cell typing and indicator detection plans, please refer to the Phenotyping with panel design and data.
Cases of Panel Design
Detection of T/B/NK (4-color) in human peripheral blood
Panel Design
| Purpose | Sample | Antibody Collocation |
|---|---|---|
| Adjust the voltage | 1 | Blank |
| Adjust compensation | 2 | CD45-PerCP |
| 3 | CD3-FITC | |
| 4 | CD16-PE / CD56-PE | |
| 5 | CD19-APC | |
| PE-FMO in combination with Isotype Control for auxiliary gating | 6 | CD45-PerCP, CD3-FITC, CD19-APC, Mouse IgG1, κ Isotype Control-PE |
| Full panel | 7 | CD45-PerCP, CD3-FITC, CD16-PE, CD56-PE, CD19-APC |
- 1. This panel has obvious cell populations, and compensation regulation can be performed without single positive tube. But for beginners of Flow Cytometry, it is recommended to set up a single positive tube to adjust compensation.
- 2. The detection standard for NK cells is CD3-CD16+CD56+.
- 3. In this panel, it is recommended to set Isotype Control for CD16 and CD56, while other indicators can be omitted due to obvious populations.
- 4. It is recommended to stain human peripheral blood samples with CD45, which is beneficial for the lymphocyte phylum gating through CD45 and SSC. It is recommended to use the single positive tube of CD45 to start the machine at low speed and set the threshold.
Treg (3-color) detection of mouse spleen
Panel Design
| Purpose | Sample | Antibody Collocation |
|---|---|---|
| Adjust the voltage | 1 | Blank |
| Adjust compensation | 2 | CD4-FITC |
| 3 | CD25-APC | |
| 4 | Foxp3-PE | |
| APC-FMO in combination with Isotype Control for auxiliary gating | 5 | CD4-FITC, Foxp3-PE, Rat IgG1, κ Isotype Control- APC |
| PE-FMO in combination with Isotype Control for auxiliary gating | 6 | CD4-FITC, CD25-APC. Mouse IgG1, κ Isotype Control-PE |
| Full Panel | 7 | CD4-FITC, CD25-APC, Foxp3-PE |
- 1. Mouse Treg marker is CD4+ CD25+ Foxp3+.
- 2. CD4 cell population is obvious, and there is no need of Isotype Control. But CD25 and Foxp3 populations are not obvious, and Isotype Controls are needed.
- 3. There is fluorescence spillover, so it is necessary to set single positive tubes for compensation.
- 4. Inappropriate use of Fixation/Permeabilization buffer may cause high background and unclear cell clustering. Please be careful.
Detection of Th17 (3-color) in human peripheral blood PBMC
Panel Design
| Purpose | Sample | Antibody Collocation |
|---|---|---|
| Adjust the voltage | 1 | Blank |
| Adjust compensation | 2 | CD3-PerCP/Cyanine5.5 |
| 3 | CD4-FITC | |
| 4 | IL-17A PE | |
| PE-FMO in combination with Isotype Control for auxiliary gating | 5 | CD3-PerCP/Cyanine5.5, CD4-FITC, Rat IgG1, κ Isotype Control-PE |
| Full panel | 6 | CD3-PerCP/Cyanine5.5, CD4-FITC, IL-17A- PE |
- 1. After PBMC sorting, it is necessary to first use cytokine stimulating and blocking agents for stimulating and blocking culture (The reagents used in this experiment are: Cytokine Activation and Protein Blocking Kit (E-CK-A091). The cultivation conditions are as follows: after 1 hour of stimulation with a stimulating agent, add a blocking agent and incubate for another 4.5 hours), then collect cells for subsequent Flow Cytometry experiments.
- 2. PMA stimulation can cause partial endocytosis of CD4 on the surface of human T cells, so we need to choose the CD4 clone SK3 with minimal impact on endocytosis.
- 3. Isotype control for IL-17A is necessary, since the expression of cytokines is generally not high.
- 4. CD3+CD4+ IL-17A+ is Th17 type.
- 5. The Permeabilization buffer may cause significant damage to cells, so it is recommended that the cell precipitates formed after centrifugation be dispersed into cell suspensions before adding the Permeabilization buffer to reduce cell damage.
Related Services
Elabscience® offers more than 3,000 high-quality Flow Cytometry Antibodies with a variety of dyes and a wide range of intracellular and extracellular markers for your selection, meeting the requirements of your flow cytometry experiment panel design.
Elabscience® can provide you with Labeling Services such as different fluorochrome labeling, biotin labeling, and HRP labeling. Please fill out the Labeling Service Request Table.
