Cell Metabolism
Cell Metabolism FAQs
In order to provide better customer support ,Elabscience have compiled and published the most frequently asked questions (FAQs) about cell metabolism assay kit, and will continue to update
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The activity center of SOD is metal ions, and the use of EDTA as an anticoagulant will chelate metal ions, which may cause the decrease of SOD enzyme activity and affect the results.
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SDS in RIPA lysis buffer interferes with enzyme activity determination, so it is not recommended to use RIPA lysis buffer for cell treatment. The SOD kit requires that the sample should not contain SDS, Tween20, NP-40, Triton X-100 and other detergents, and should not contain DTT, 2-mercaptoethanol and other reducing reagents.
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The environment, operation and other influencing factors of each experiment will be a little different. It is recommended to re-examine the control well and control blank well in each experiment in order to obtain more accurate results.
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It is recommended that cell suspensions that have been homogenized be tested on the same day and not frozen.If there is a temporary accident, you can directly freeze the cells, it is generally recommended that -80℃ not exceed one month.
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Yes, as long as the temperature can reach 100℃ and the heat is uniform.
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The control well is mainly set to eliminate the interference of the sample itself. If there is hemolysis or lipemia in the blood sample, it is necessary to set the control well; if there is no such phenomenon, it is not necessary to set the control well.
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The sample homogenate medium of this kit can only be used with reagent 5 as required in the manual, and cannot be replaced.
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It is normal that solid precipitation may occur after storage of reagent 3. It is heated and dissolved at 80℃, and can be used normally after cooling.
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It is a normal phenomenon. After taking it out from -20℃, it can be used after thawing at room temperature.
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None of the microplate in the metabolism kit can be reused.If the microplate in this kit is not enough, you can use the new black microplate in your own laboratory instead.
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The kit must be detected by an instrument with chemiluminescence function. Without a chemiluminescence instrument, a multi-function microplate reader with chemiluminescence function can be used, and a ordinary microplate reader cannot be used.When the kit is tested, it only needs to set the chemiluminescence detection, but does not need to set the excitation and emission wavelength.
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It is recommended to use fresh samples as far as possible. If fresh samples are not available, it is recommended that the samples be frozen at -80℃ for no more than one month, otherwise the AST/ALT enzyme activity will decrease, which will have a great impact on the results.
