A Reliable Research Partner in Life Science and Medicine

In experiments that involve living cells or animals—particularly those aimed at elucidating the biological activity of the protein/antibody per se—the use of sterile and low endotoxin reagents is strongly advised.

Storage Conditions: Store antibodies at 2–8°C, protected from light. Do not freeze unless specifically instructed. Both fluorochromes and antibody proteins are highly sensitive to freeze-thaw cycles; repeated freezing and thawing can lead to protein denaturation, fluorescence quenching, or aggregate formation. For long-term storage, consider aliquoting into smaller volumes to avoid repeat handling. Transport Effects: Short-term ambient temperature transport generally does not significantly compromise antibody titer or performance. However, if stored at room temperature for longer than one week, antibody efficacy may decline. Handling Recommendation: Upon receipt, briefly centrifuge the vial to collect any liquid from the cap and walls into the bottom of the tube to prevent loss during pipetting.

ELISA testing is characterized by high sensitivity, strong specificity, simple operation, small sample volume, and high-throughput detection. It also offers high accuracy and good repeatability in its results, making it widely used in clinical and research settings. When choosing a high-quality ELISA kit, the following points should be considered: 1. Research purpose and ELISA type. Determine whether the ELISA kit is for research, clinical diagnosis, or other applications. Select the appropriate ELISA kit type based on your specific experimental needs and target characteristics. 2. Sample type and sample volume. Ensure that the kit can detect your sample type and that the required sample volume is suitable for the available sample volume, as some kits may require a larger volume for accurate measurement. 3. Pay attention to the performance parameters of the kit (sensitivity, specificity, precision, stability, recovery rate), and choose a high-quality ELISA kit. 4. Convenience of experimental operation. Under the condition of ensuring quality, the shorter the operation time and the more convenient the operation of the kit, the better.

The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer.
On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.