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96T $ 420.00
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For research use only.

Detection Principle Histamine is an endogenous active substance that exists widely in nature and is an important mediator involved in inflammatory response and immune damage. The histamine content increases with the degree of spoilage of the organism. Histamine can produce chromogenic substances with chromogenic agents under the action of enzymes, which has a maximum absorption peak at 460 nm. The histamine content of the sample can be calculated by measuring the OD value at 460 nm.
Sample Type Serum,Plasma,Animal tissue,Plant tissue,Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (440-480 nm, optimum wavelength: 460 nm)
Research Area Amino Acids And Proteins
Other Reagents Required Double distilled water
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 2.44 μmol/L
Detection Range 2.44-200 μmol/L
Precision inter-assay CV: 0.5-1.6% | intra-assay CV: 1.5-4.2%
Assay Time 1 h 10 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Fresh salmon tissue homogenate 1
10% Pelteobagrus fulvidraco tissue homogenate 1
10% Spanish mackerel tissue homogenate 1
10% Spoiled Spanish mackerel tissue homogenate 1
10% Thunnini tissue homogenate 3-6
10% Spoiled Thunnini tissue homogenate 4-10
10% Pepper tissue homogenate 2-8
10% Grape flesh tissue homogenate 1
10% Garlic tissue homogenate 1
10% jujube tissue homogenate 2-8
Rat serum 1
Mouse serum 1
Fetal calf serum 1
0.2 × 10^6 Jurkat cell 1
0.2 × 10^6 4T1 cell 1
0.2 × 10^6 HL-60 cell 1
0.2 × 10^6 293T cell 1
0.2 × 10^6 CHO cell 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor®405

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

detection instrument

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