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Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit

Cat:E-CL-H0071
Manual MSDS

Price: $ 682

Price: $ 546

Price: $ 180

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Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Human
  • Detection Range: 12.5-800 pg/mL
  • Sensitivity: 7.5 pg/mL
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Product Details
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AFGF/FGF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AFGF/FGF1. You can calculate the concentration of Human AFGF/FGF1 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Chemiluminescence
Detection Range 12.5-800 pg/mL
Sensitivity 7.5 pg/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Human AFGF/FGF1 in samples.No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Human AFGF/FGF1 concentrations in serum, plasma and other biological fluids.
Applications CLIA
Storage 2-8℃/-20℃
Database SwissProt:  P05230
Syonoyms FGF-1,ECGF,ECGF-beta,A-FGF,FGFA,ECGFA,ECGFB,FGF-Alpha,HBGF1,HBGF-1,ECGFB,GLIO703,Heparin-binding growth factor 1
Research Area Cancer Cardiovascular Neuroscience Signal Transduction