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Mouse SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

  • Cat.No.:E-EL-M2398

  • Reactivity: Mouse

To Purchase E-EL-M2398

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.50 3.60 0.50 1.40 3.30
Standard deviation 0.03 0.08 0.19 0.03 0.07 0.11
CV (%) 6.00 5.33 5.28 6.00 5.00 3.33

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 100
EDTA plasma(n=8) 89-101 95
Cell culture media(n=8) 90-101 96

Linearity

Samples were spiked with high concentrations of Mouse SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P08228
SynonymsALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research AreaCancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Physiology (2022) IF: 4.755
    Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

    DOI: 10.3389/fphys.2022.984429

    PMID: 36091371

    Sample: Lung,Serum,Testis
  2. Molecular Medicine Reports (2018) IF: 1.922
    Embelin inhibits abdominal aortic aneurysm through decreasing IL‑6‑induced STAT3 and NF‑κB inactivation

    DOI: 10.3892/mmr.2018.9221

    Sample: Aortic Tissues
  3. Molecular Medicine Reports (2020) IF: 2.1
    Coenzyme Q10 alleviates sevoflurane‑induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons

    DOI: 10.3892/mmr.2020.11131

    Sample: Tissue Homogenate
  4. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019) IF: 2.11
    The effects of spinosad on antioxidant system and cognitive performance of mice

    DOI: 10.1080/13813455.2019.1623264

    Sample: Hippocampus,Prefrontal Cortex Tissue
  5. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Combination of endothelial progenitor cells and BB‑94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke

    DOI: 10.3892/etm.2021.10221

    Sample: Cell Culture Supernatant
  6. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557

    Sample: Small Intestinal Tissues
  7. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Apigenin alleviates methotrexate-induced liver and kidney injury in mice:

    DOI: 10.1177/09603271211009964

    Sample: Whole Blood,Tissue Homogenate
  8. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro

    DOI: 10.1007/s12640-019-00114-x

    Sample: Mouse Astrocyte Cell
  9. PHARMACEUTICAL BIOLOGY (2023) IF: 3.889
    Antihyperglycemic effects of Lysiphyllum strychnifolium leaf extract in vitro and in vivo

    DOI: 10.1080/13880209.2022.2160771

    Sample: Muscle
  10. INFLAMMATION (2022) IF: 4.092
    TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT–GSK-3β Pathway

    DOI: 10.1007/s10753-022-01638-9

    Sample: Podocytes
  11. NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
    Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro

    DOI: 10.1016/j.neuint.2022.105405

    PMID: 35934159

    Sample: Astrocyte Cell
  12. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes

    DOI: 10.1016/j.biopha.2020.110756

    Sample: Serum
  13. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

    DOI: 10.1016/j.procbio.2022.11.001

    Sample: Serum,Liver
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