Click here to view cell apoptosis assay products
×

Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

  • Cat.No.:E-EL-M1116

  • Reactivity: Mouse

To Purchase E-EL-M1116

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse TRACP-5b in samples. No significant cross-reactivity or interference between Mouse TRACP-5b and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse TRACP-5b concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRACP-5b. You can calculate the concentration of Mouse TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TRACP-5b were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TRACP-5b were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.52 5.33 22.43 2.70 4.86 21.67
Standard deviation 0.15 0.26 1.11 0.18 0.24 0.75
CV (%) 5.95 4.88 4.95 6.67 4.94 3.46

Recovery

The recovery of Mouse TRACP-5b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 99
EDTA plasma (n=8) 89-102 96
Cell culture media (n=8) 93-103 98

Linearity

Samples were spiked with high concentrations of Mouse TRACP-5b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q05117
Research AreaCancer, Metabolism, Immunology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of the American Chemical Society (2020) IF: 14.612
    Smart Nanosacrificial Layer on the Bone Surface Prevents Osteoporosis through Acid–Base Neutralization Regulated Biocascade Effects

    DOI: 10.1021/jacs.0c07309

    PMID: 32960592

    Sample: Serum
  2. Redox Biology (2021) IF: 11.799
    Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis

    DOI: 10.1016/j.redox.2021.101915

    PMID: 33662874

    Sample: Serum
  3. Acta Biomaterialia (2022) IF: 10.633
    Genesis of osteoclasts on calcium phosphate ceramics and their role in material-induced bone formation

    DOI: 10.1016/j.actbio.2022.11.005

  4. CELL PROLIFERATION (2023) IF: 8.7550
    Dietary nitrate improves jaw bone remodelling in zoledronate-treated mice

    DOI: 10.1111/cpr.13395

    Sample: Plasma,tooth sockets
  5. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Thiaplakortone B attenuates RANKL‐induced NF‐κB and MAPK signaling and dampens OVX‐induced bone loss in mice

    DOI: 10.1016/j.biopha.2022.113622

    Sample: serum
  6. Oncotarget (2017) IF: 5.168
    Dectin-1 signaling inhibits osteoclastogenesis via IL-33-induced inhibition of NFATc1

    DOI: 10.18632/oncotarget.18411

    Sample: Bone marrow cells (BMCs)
  7. Bone & Joint Research (2022) IF: 5.853
    N,N-Dimethylformamide inhibits high glucose-induced osteoporosis via attenuating MAPK and NF-κB signalling

    DOI: 10.1302/2046-3758.114.BJR-2020-0308.R2

    Sample: plasma
  8. JOURNAL OF BONE AND MINERAL RESEARCH (2020) IF: 5.854
    Hymenialdisine: A Marine Natural Product That Acts on Both Osteoblasts and Osteoclasts and Prevents Estrogen-Dependent Bone Loss in Mice

    DOI: 10.1002/jbmr.4025

    Sample: serum
  9. STEM CELLS (2016) IF: 5.902
    Brief Report: Consecutive Alendronate Administration‐Mediated Inhibition of Osteoclasts Improves Long‐Term Engraftment Potential and Stress Resistance of HSCs

    DOI: 10.1002/stem.2425

    Sample: Serum
  10. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice

    DOI: 10.3390/ijms22179590

    PMID: 34502499

    Sample: plasma
  11. Aging and Disease (2019) IF: 4.232
    Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

    DOI: 10.14336/AD.2018.0921

    Sample: serum
  12. BONE (2021) IF: 4.398
    Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

    DOI: 10.1016/j.bone.2021.116211

    PMID: 34560308

    Sample: serum
  13. CHEMICO-BIOLOGICAL INTERACTIONS (2017) IF: 3.143
    Methoxsalen supplementation attenuates bone loss and inflammatory response in ovariectomized mice

    DOI: 10.1016/j.cbi.2017.10.014

    Sample: Serum
  14. Journal of Ayurveda and Integrative Medicine (2018)
    Eurycoma longifolia, a promising suppressor of RANKL-induced differentiation and activation of osteoclasts: An in vitro mechanistic evaluation

    DOI: 10.1016/j.jaim.2017.07.014

    Sample: RAW 264.7 cells
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha: