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NGF Polyclonal Antibody

Cat:E-AB-40051
Manual MSDS

Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Hela,Raji
Dilution

WB 1:500-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant human Beta-nerve growth factor protein
Abbre NGF
Synonyms Beta nerve growth factor;Beta NGF;Beta-nerve growth factor;Beta-NGF;HSAN5;MGC161426;MGC161428;Nerve growth factor (beta polypeptide);Nerve growth factor;Nerve growth factor beta;Nerve growth factor beta polypeptide;Nerve growth factor beta subunit;NGF;NGF;NGFB;NID67
Swissprot
Calculated MW 27kDa
Observed MW 27kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted.
Concentration 2mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer; Neuroscience
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.