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For research use only.

Verified Samples Verified Samples in WB: Mouse lung, Rat lung
Verified Samples in IHC: Human tonsil, Rat spleen
Dilution WB 1:500-1:2000,  IHC 1:500-1:1000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen Recombinant Human CD38 protein expressed by E.coli
Abbre CD38
Synonyms ADP ribosyl cyclase,   ADPRC1,   CD38 antigen,   CD38 molecule,   CD38H,   Cyclic ADP ribose hydrolase,   EC 3.2.2.5,   I-19,   NIMR5 antigen,   T10,   p45,  Acute lymphoblastic leukemia cells antigen CD38
Swissprot
Calculated MW 34 kDa
Observed MW 45 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Affinity purification
Research Areas Immunology,  Stem Cells,  Cancer,  Metabolism
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background CD38(cluster of differentiation38), also known ascyclic ADP ribose hydrolaseis aglycoproteinfound on the surface of many immune cells (white blood cells), includingCD4+,CD8+,B lymphocytesandnatural killer cells. CD38 also functions incell adhesion,signal transductionandcalcium signaling. In humans, the CD38 protein is encoded by the CD38 gene which located on chromosome 4.
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Unconjugated

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