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ELISA Curve Fitting Calculation Tool

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Step1:Data Input

*
*
*
*
*

(Including blank wells)

X (Concentration) Y1 (Measured Value) Y2 (Measured Value) Y3 (Measured Value)
{{rowIndex+1}}

Step2:Data Fitting

Four Parameter Logistic Fit

Regression equation and parameters

Equation: y=(A-D)/[1+(x/C)^B]+D
Parameter a = {{A_data}}
Parameter b = {{B_data}}
Parameter c = {{C_data}}
Parameter d = {{D_data}}
R²= {{R_data}}

Step3:Calculate Values

Y(Measured Value) Input value Y(Adjusted Value)Auto-calculated X(Calculated Concentration)Auto-calculated X(Concentration)Auto-calculated
{{rowIndex+1}} {{row.y2}} {{row.x1}} {{row.x2}}
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