ELISPOT Kits
The ELISPOT Kit is based on the principle of the ELISA sandwich method, combined with cell culture technology. It uses pre-coated capture antibodies to capture cytokines secreted by cells, and the enzyme-labeled secondary antibody produces color spots, allowing for functional detection at the single-cell level. ELISPOT offers ultra-high sensitivity (capable of detecting active cells as rare as one in a million), single-cell resolution, visualization, and is easy and efficient to operate.
The ELISPOT Kit is an ideal tool for studying cellular immune function, precisely evaluating T/B cell activity. It is widely used in vaccine development, tumor immunotherapy, research on autoimmune disease mechanisms, and the diagnosis and efficacy monitoring of infectious diseases.
View All ELISPOT Kits
Why Choose ELISPOT Kits
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Ultra-high Sensitivity
Capable of detecting active cells at a sensitivity of 1 in 1,000,000
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Single-cell Resolution
Direct visualization of single-cell function for precise quantification of immune activity
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High Stability
Intra- and inter-batch CV<10%, with clear and easy-to-read spots and high data reproducibility
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Ready-to-use
Pre-coated plates are easy to handle; analysis can be conducted with a microscope or plate reader
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Multi-sample Compatibility
Supports various samples such as PBMCs, spleen cells, and tumor-infiltrating lymphocytes (TILs)
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Cost-effective
Fast delivery and a cost-effective choice
Intra-and inter-batch variation testing results for various cytokines.
Detection results for the secretion of human IFN-γ and IL-1β by various cell types.
Application Case Studies
Product List
| Cat. No. | Product Name | Size |
|---|---|---|
| ESP-H0002 | Human IFN-γ (Interferon Gamma) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0005 | Human IL-1β (Interleukin 1β) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0006 | Human IL-2 (Interleukin 2) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0007 | Human IL-4 (Interleukin 4) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0008 | Human IL-5 (Interleukin 5) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0009 | Human IL-6 (Interleukin 6) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0003 | Human IL-10 (Interleukin 10) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0004 | Human IL-17A (Interleukin 17A) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0010 | Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit | 96 T*1/96 T*5 |
| Cat. No. | Product Name | Size |
|---|---|---|
| ESP-M0001 | Mouse IFN-γ (Interferon Gamma) ELISPOT Kit | 96 T*1/96 T*5 |
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