NA/NE(Noradrenaline/Norepinephrine) ELISA Kit (E-EL-0047)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal NA/NE. During the reaction, Universal NA/NE in the sample or standard competes with a fixed amount of Universal NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Norepinephrine (INN name: Norepinephrine, also known as Noradrenaline, abbreviated NE or NA), also known as norepinephrine, is a substance formed after the removal of N-methyl group of adrenaline, and also belongs to catecholamines in chemical structure. It is both a neurotransmitter, mainly synthesized and secreted by sympathetic postganglionic neurons and adrenergic nerve endings in the brain, which is the main transmitter released by the latter, and a hormone, synthesized and secreted by the adrenal medulla, but in smaller amounts.