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8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

  • Cat.No.:E-EL-0028

  • Reactivity: Universal

To Purchase E-EL-0028

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes 8-OHdG in samples. No significant cross-reactivity or interference between 8-OHdG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-OHdG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-OHdG were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.79 10.11 46.98 4.48 9.61 48.89
Standard deviation 0.27 0.58 1.69 0.27 0.55 1.91
CV (%) 5.66 5.69 3.60 5.95 5.70 3.90

Recovery

The recovery of 8-OHdG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 91
EDTA plasma(n=8) 91-104 98
Cell culture media(n=8) 93-106 98

Linearity

Samples were spiked with high concentrations of 8-OHdG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms7,8-Dihydro-8-oxo-2'-deoxyguanosine; 7,8-Dihydro-8-oxodeoxyguanosine; 8-Hydroxy-2'-deoxyguanosine; 8-Hydroxydeoxyguanosine; 8-Oxo-2'-deoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 8-Oxo-7,8-dihydrodeoxyguanosine; 8-Oxo-dG; 8-OH-dG

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nature (2020) IF: 42.778
    DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25

    DOI: 10.1038/s41586-020-2394-6

    PMID: 32528174

    Sample: DNA
  2. Frontiers in Immunology (2017) IF: 6.429
    Chronic Obstructive Pulmonary Disease-Derived Circulating Cells Release IL-18 And IL-33 Under Ultrafine Particulate Matter Exposure In A Caspase-1/8-Independent Manner.

    DOI: 10.3389/fimmu.2017.01415

    PMID: 29123531

    Sample: Cell culture supernatant
  3. Environmental Research (2020) IF: 5.715
    Effect of acute exposure of saxitoxin on development of zebrafish embryos (Danio rerio)

    DOI: 10.1016/j.envres.2020.109432

    PMID: 32247151

    Sample: Tissue homogenate
  4. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Nodularin induced oxidative stress contributes to developmental toxicity in zebrafish embryos

    DOI: 10.1016/j.ecoenv.2020.110444

    PMID: 32169726

    Sample: Tissue homogenate
  5. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Dibutyl phthalate aggravated asthma-like symptoms through oxidative stress and increasing calcitonin gene-related peptide release

    DOI: 10.1016/j.ecoenv.2020.110740

    PMID: 32446102

    Sample: Tissue homogenate
  6. Environmental Research (2019) IF: 4.732
    The impact on T-regulatory cell related immune responses in rural women exposed to polycyclic aromatic hydrocarbons (PAHs) in household air pollution in Gansu, China: A pilot investigation

    DOI: 10.1016/j.envres.2019.03.053

    PMID: 30951957

    Sample: Urine
  7. International Journal of Nanomedicine (2016) IF: 4.3
    Cerium Oxide Nanoparticles Promote Neurogenesis And Abrogate Hypoxia-Induced Memory Impairment Through AMPK-PKC-CBP Signaling Cascade.

    DOI: 10.2147/IJN.S102096

    PMID: 27069362

    Sample: Tissue homogenate
  8. Environmental Geochemistry and Health (2020) IF: 3.472
    Blood lead and cadmium levels in occupationally exposed workers and their effect on markers of DNA damage and repair

    DOI: 10.1007/s10653-020-00696-y

    PMID: 32813162

    Sample: Plasma
  9. Psychiatry and clinical neurosciences (2019) IF: 3.351
    Increased plasma levels of 8-oxoguanine DNA glycosylase-1 in bipolar disorder

    DOI: 10.1111/pcn.12928

    PMID: 31482650

    Sample: Plasma
  10. Journal of cellular physiology (2019) IF: 3.923
    Thioredoxin-interacting Protein Deficiency Alleviates Phenotypic Alterations of Podocytes via Inhibition of mTOR Activation in Diabetic Nephropathy

    DOI: 10.1002/jcp.28317

    PMID: 30746698

    Sample: Urine
  11. Frontiers in Pharmacology (2019) IF: 3.831
    AIM2 Inflammasome Activation Leads to IL-1α and TGF-β Release From Exacerbated Chronic Obstructive Pulmonary Disease-Derived Peripheral Blood Mononuclear Cells

    DOI: 10.3389/fphar.2019.00257

    PMID: 30930781

    Sample: Cell culture supernatant
  12. Biological Trace Element Research (2020) IF: 2.639
    Evaluation of DNA Damage and Expressions of DNA Repair Gene in Occupationally Lead Exposed Workers (Jodhpur, India)

    DOI: 10.1007/s12011-020-02298-2

    PMID: 32712906

    Sample: Serum
  13. PloS one (2020) IF: 2.74
    Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO and caspase 3

    DOI: 10.1101/796110

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  14. PloS one (2020) IF: 2.74
    Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO/TNF-α and caspase 3 activities

    DOI: 10.1371/journal.pone.0224052

    PMID: 32168344

    Sample: Serum,Tissue homogenate
  15. Redox Report (2020) IF: 2.753
    Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

    DOI: 10.1080/13510002.2020.1752003

    PMID: 32290793

    Sample: Serum,Tissue homogenate
  16. J TRACE ELEM MED BIO (2020) IF: 2.895
    Possible differences in the mechanism of malignant transformation of HaCaT cells by arsenite and its dimethyl metabolites, particularly dimethylthioarsenics

    DOI: 10.1016/j.jtemb.2020.126544

    PMID: 99999999

    Sample: Cell culture supernatant
  17. Plos One (2017) IF: 2.806
    A Comparative Study On Antioxidant System In Fish Hepatopancreas And Intestine Affected By Choline Deficiency: Different Change Patterns Of Varied Antioxidant Enzyme Genes And Nrf2 Signaling Factors.

    DOI: 10.1371/journal.pone.0169888

    PMID: 28099509

    Sample: Tissue homogenate
  18. Andrologia (2019) IF: 1.588
    LY294002, a PI3K pathway inhibitor, prevents leptin-induced adverse effects on spermatozoa in Sprague-Dawley rats

    DOI: 10.1111/and.13196

    PMID: 30456785

    Sample: Tissue homogenate
  19. Journal of Essential Oil Bearing Plants (2019) IF: 0.824
    The Protective Effects of Thymol Against Ketoprofen Induced Damages on Pancreatic Acinar and Islet of Langerhans Cells in Rats

    DOI: 10.1080/0972060X.2019.1625814

    PMID: 99999999

    Sample: Tissue homogenate
  20. Eurasian Journal of Biosciences (2020)
    An exploration of the relationship between interleukin 37, 8-ohdg and a number of anthropometric measurements in iraqi rheumatoid arthritis patients

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  21. Meta Gene (2020)
    Effect of exercise, synthetic anabolic steroids and protein intake on DNA damage in trained and untrained men

    DOI: 10.1016/j.mgene.2020.100685

    PMID: 99999999

    Sample: Serum
  22. Journal of Pediatric Hematology/Oncology (2020)
    Clinical Laboratory Manifestation and Molecular Diagnosis of β-Thalassemia Patients in Iraq

    DOI: 10.1097/MPH.0000000000001656

    PMID: 31714438

    Sample: Serum
  23. Available at SSRN (2018)
    Hypothermic Oxygenated Machine Perfusion Alleviates Donation after Cardiac Death Liver Injury Through Regulating P-Selectin-Dependent and-Independent Pathways in Mice

    DOI: 10.1097/TP.0000000000002621

    PMID: 31033856

    Sample: Tissue homogenate
  24. Environmental Health Insights (2019)
    Impairment of Mitochondrial-Nuclear Cross Talk in Lymphocytes Exposed to Landfill Leachate

    DOI: 10.1177/1178630219839013

    PMID: 31168291

    Sample: Cell culture supernatant
  25. International Journal of Scientific & Technology research (2018) IF: /
    Toxicological Studies On Heavy Metal Bioaccumulation And Oxidative DNA Damage In Residents Of An Oil Producing Area.
    Sample: Serum
  26. Medicine Science (2018)
    Elevated Serum Levels Of 8-Hydroxy-2-Deoxyguanosine In Mild-Moderate Acne Vulgaris.

    DOI: 10.5455/medscience.2017.06.8705

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