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AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]

  • Cat.No.:E-AB-F11030

  • Applications: FCM,Activ
  • Isotype: Armenian Hamster IgG
  • Host: Armenian Hamster

To Purchase E-AB-F11030

Size:
  • 50μg
  • 500μg
  • 1mg
Price: $75
Qty:

Antigen Details

Alternate Names T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E
Swissprot P22646
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form Liquid
Clone No. 145-2C11
Host Armenian Hamster
Isotype Armenian Hamster IgG
Isotype Control AF/LE Purified Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F098530]
Reactivity Mouse
Application FCM,Activ
Storage Buffer 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
Purified Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
Biotin Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
PE Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
APC Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11] 25μg, 100μg Check More

Citations

  1. INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 3.361
    Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages

    DOI: 10.1016/j.intimp.2019.106012

    Sample: Cell Sample

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