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APC Anti-Mouse CD62L Antibody[Mel14]

  • Cat.No.:E-AB-F1011UE

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1011UE

Size:
  • 25μg
  • 100μg
Price: $60
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Antigen Details

Alternate Names L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22
Swissprot P18337
References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Product Details

Form Liquid
Clone No. Mel14
Host Rat
Isotype Rat IgG2a, κ
Isotype Control APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833E]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and nave T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte 'rolling' on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish nave, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD62L Antibody[Mel14] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
Biotin Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
FITC Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD62L Antibody[Mel14] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
PE Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD62L Antibody[Mel14] 25μg, 100μg Check More

Citations

  1. ADVANCED MATERIALS (2022) IF: 32.086
    Lymph Node Targeted Drug Delivery for Effective Immunomodulation to Prolong the Long-Term Survival After Heart Transplantation

    DOI: 10.1002/adma.202207227

    PMID: 36314402

    Sample: Lymph Nodes,Spleens

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