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For research use only.

Verified Samples Verified Samples in WB: 22Rv1, HT29, L-O2, HeLa, Mouse liver
Dilution WB 1:1000-1:3000
Isotype IgG
Host Rabbit
Reactivity Human,  Rat
Applications WB
Clonality Polyclonal
Immunogen Recombinant fusion protein of human AVEN (NP_065104.1).
Abbre AVEN
Synonyms AVEN,  PDCD12
Swissprot
Calculated MW 38 kDa
Observed MW 55 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Endomembrane system. Associated with intracellular membranes.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Cell Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background AVEN, a cell death regulator, is an important signal inducer in acute leuke-mias. AVEN is a highly conserved peripheral membrane protein that protects the cell against the proteolytic activation of caspases as well as Apaf-1 mediated apoptosis by interfering with Apaf-1’s ability to self-associate. Bcl-2 and Bad also interact with AVEN to prevent apoptosis. AVEN is highly expressed in ovary, heart, thymus, spleen, testis and colon, but can also be detected in other tissues. Erythropoietin and methylprednisolone may play important roles in the expression of AVEN in cardiac tissue, especially after a traumatic brain injury. In young patients suffering from acute lymphoblastic leukemia (ALL), AVEN expression may be a useful tool in prognosis prediction.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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