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β - Galactosidase (β –GAL) Activity Assay Kit

Cat.No.:E-BC-K631-M
Detection instrument: Microplate reader (390-410 nm,optimum wavelength: 400 nm)

Detection method: Colorimetric method

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96T

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Detection principle

β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm.

Performance characteristics

Synonyms	β-GAL
Sample type	serum , plasma, cells, animal and plant tissue
Sensitivity	16.19 U/L
Detection range	16.19-1200 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3%
Other instruments required	Water bath, Incubator, Centrifuge, Microplate reader
Other reagents required	2 mmol/L hydrochloric acid
Storage	Reagent 2: -20℃; Others: 2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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