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c-Kit Polyclonal Antibody

Cat:E-AB-70340 Citations (2)
Manual MSDS

Price: $ 410

Price: $ 260

Price: $ 160

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Mouse skin,293T,CaCo-2,Mouse Lung,Mouse Skin,Rat skin
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant protein corresponding to Mouse c-Kit
Abbre c-Kit
Synonyms C Kit;c-Kit;c-Kit Ligand;CD117;Kit;Kit Ligand;KIT oncogene;KIT proto oncogene receptor tyrosine kinase;KIT;Mast cell growth factor receptor;Mast/stem cell growth factor receptor Kit;MGF;p145 c-kit;PBT;Piebald trait protein;Proto oncogene c Kit;Proto oncogene tyrosine protein kinase Kit;Proto-oncogene c-Kit;SCF Receptor;SCFR;soluble KIT variant 1;Steel Factor Receptor;Stem cell factor receptor;tyrosine protein kinase Kit;Tyrosine-protein kinase Kit;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Swissprot
Calculated MW 110kDa
Observed MW 145kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane, Cytoplasm, Membrane
Concentration 3 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Developmental Biology; Immunology; Neuroscience; Signal Transduction; Stem Cells; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.