ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+ -ATPase activity was inhibited, while in the sample system, Ca2+ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+ -ATPase during the incubation time. The activity of Ca2+ -ATPase was determined by inorganic phosphorus production.

Performance characteristics

Synonyms	Ca2+-ATP酶
Sample type	animal tissue
Sensitivity	1.18 U/kg wet weight
Detection range	1.18-286.43 U/kg wet weight
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	90 min
Precision	Average inter-assay CV: 8%Average intra-assay CV: 6%
Other instruments required	Test tube, Vortex mixer, Incubator, Centrifuge, 100°C water bath, Microplate reader
Other reagents required	Ultrapure water, Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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