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Ca2+-ATPase Activity Assay Kit

  • Cat.No.:E-BC-K212-M

  • Detection instrument: Microplate reader (660 nm)

To Purchase E-BC-K212-M

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Price: $260

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Detection principle

ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+ -ATPase activity was inhibited, while in the sample system, Ca2+ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+ -ATPase during the incubation time. The activity of Ca2+ -ATPase was determined by inorganic phosphorus production.

Performance characteristics

Synonyms Ca2+-ATP酶
Sample type animal tissue
Sensitivity 1.18 U/kg wet weight
Detection range 1.18-286.43 U/kg wet weight
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 90 min
Precision Average inter-assay CV: 8%Average intra-assay CV: 6%
Other instruments required Test tube, Vortex mixer, Incubator, Centrifuge, 100°C water bath, Microplate reader
Other reagents required Ultrapure water, Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months


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