Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit (E-BC-K834-M)
For research use only.
| Detection Principle | Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of mitochondrial complex I can be quantified by measure the change OD value at 340 nm. |
| Synonyms | NADH-CoQ Reductase |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Mitochondria Function , Energy Metabolism |
| Other Reagents Required | Anhydrous ethanol (AR) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.85 U/L |
| Detection Range | 2.85-119.04 U/L |
| Precision | inter-assay CV: 5.0-8.0% | intra-assay CV: 3.0-4.0% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Jurkat cell | 1 |
| CHO cell | 1 |
| HL-60cell | 1 |
| Hela cell | 1 |
| 293T cell | 1 |
| Molt-4 cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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