ATP Chemiluminescence Assay Kit (E-BC-F002)
For research use only.
Detection Principle | Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range. |
Synonyms | ATP |
Sample Type | Animal tissue,Cell |
Detection Method | Fluorometric method |
Detection Instrument | Chemiluminescence detector |
Research Area | Oxidative Stress , Tricarboxylic Acid(TCA)Cycle , Mitochondria Function , Disulfidptosis , Energy Metabolism |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.003 μmol/L |
Detection Range | 0.003-5 μmol/L |
Precision | inter-assay CV: 6.5% | intra-assay CV: 2.2% |
Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Mouse heart tissue homogenate | 1 |
10% Mouse kidney tissue homogenate | 1 |
10% Mouse muscle tissue homogenate | 1 |
10% Mouse liver tissue homogenate | 1 |
10% Mouse brain tissue homogenate | 1 |
10% Mouse lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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