CK-19 Polyclonal Antibody (E-AB-70083)
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For research use only.
| Verified Samples |
Verified Samples in WB: A431, A549, HepG2, MCF-7, SW480, HT29, Human colon cancer, Mouse lung, Rat lung Verified Samples in IHC: Human liver, Mouse kidney, Rat kidney, Human Small intestine |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:800 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 19 |
| Abbre | CK-19 |
| Synonyms | 40 kDa keratin intermediate filament, 40 kd, CK 19, CK-19, CK19, Cytokeratin 19, Cytokeratin-19, K19, K1C19, K1CS, KRT19, Keratin, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin-19, MGC1, type I, type I cytoskeletal 19 |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
44 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.66 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19,one of type I keratins,is specifically expressed in the periderm,the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity,KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes,peripheral blood,and bone marrow of breast cancer patients. This antibody,generated against full length KRT19 protein,also recognizes KRT17,another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma. |
Other Clones
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Other Formats
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Unconjugated
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