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CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat.No.:E-BC-K022-S
Detection instrument: Spectrophotometer(550 nm)

Detection method: Colorimetric method

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Detection principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Performance characteristics

Synonyms	CuZn-SOD,T-SOD
Sample type	Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity	2.03 U/mL
Detection range	2.03-155 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	100 min
Precision	Average inter-assay CV: 7.200%Average intra-assay CV: 4%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor	The volume added to the reaction (μL)
Mouse serum	3-5	20-30
Human serum	1	30-40
Rat serum	3-5	20-30
Rat plasma	1-2	20-30
Human hydrothorax	1	30-50
Human urine	1	30-50
10% Mouse liver tissue homogenate	50-80	20-30
10% Mouse brain tissue homogenate	8-12	20-30
10% Mouse kidney tissue homogenate	10-20	20-30
HepG2 cells (3.27 mgprot/mL)	5-10	20-30

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Nephroprotective Effect of Fennel (Foeniculum vulgare) Seeds and Their Sprouts on CCl4-Induced Nephrotoxicity and Oxidative Stress in Rats

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Sep 23 2022 ]

Asked: Can this kit detect the activity of Mn-SOD?

adminSubmitted [ Sep 23 2022 ]

Answered: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.

s************eSubmitted [ Mar 19 2019 ]

Asked: can we used these kits for microlab 300. Thank you

adminSubmitted [ Mar 19 2019 ]

Answered: I'm sorry this kit can't be used for microlab 300.

A******aSubmitted [ Jan 30 2019 ]

Asked: Kit no catalog E-BC-K022 can be used for what species? Rat, mice or human? Thank you

adminSubmitted [ Jan 30 2019 ]

Answered: Hi Agnestia, Thanks for your question. This kit Cat.# E-BC-K022 is an universal kit and can be used for rat, mice, human and other species. Please feel free to let us know if any question. Thank you.

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Asked: This kit can be used for what? rat, human, or mice? Thank you

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