Current Position:Home>>Kits>>Metabolism Assays>> CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
Cat.No.:E-BC-K022-S
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Synonyms | CuZn-SOD,T-SOD |
Sample type | Serum,plasma,urine,cells,cell culture supernatant,tissue |
Sensitivity | 2.03 U/mL |
Detection range | 2.03-155 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 100 min |
Precision | Average inter-assay CV: 7.200%Average intra-assay CV: 4% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | Reagent 4: -20℃, others: 2-8℃ |
Valid period | 12 months |
The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
The volume added to the reaction (μL) |
Mouse serum |
3-5 |
20-30 |
Human serum |
1 |
30-40 |
Rat serum |
3-5 |
20-30 |
Rat plasma |
1-2 |
20-30 |
Human hydrothorax |
1 |
30-50 |
Human urine |
1 |
30-50 |
10% Mouse liver tissue homogenate |
50-80 |
20-30 |
10% Mouse brain tissue homogenate |
8-12 |
20-30 |
10% Mouse kidney tissue homogenate |
10-20 |
20-30 |
HepG2 cells (3.27 mgprot/mL) |
5-10 |
20-30 |
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DOI: 10.1111/pce.14263 |
|
PMID: 35040151 |
Sample: leaf |
Sample: serum |
Sample: Serum,kidney tissue |
Sample: plasma |
PMID: 35453477 |
Sample: blood |
PMID: 35453353 |
Sample: serum |
Sample: serum |
DOI: 10.1155/2020/2978340 |
|
PMID: 32655765 |
Sample: Plasma,Urine,Tissue homogenate |
Sample: serum |
PMID: 36230124 |
Sample: serum |
DOI: 10.1002/lt.26421 |
|
PMID: 35100468 |
Sample: liver |
PMID: 31972360 |
Sample: Tissue homogenate |
PMID: 33310035 |
Sample: cell |
PMID: 36152772 |
Sample: brain tissue |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: myocardium |
DOI: 10.1155/2020/1936406 |
|
PMID: 32117470 |
Sample: Plasma,Tissue homogenate |
DOI: 10.1111/jphp.13319 |
|
Sample: Cell lysate |
PMID: 32689984 |
Sample: Plant Fluid |
Sample: Plasma |
DOI: 10.1111/and.13825 |
|
PMID: 32926461 |
Sample: Cell lysate |
DOI: 10.1155/2020/9046862 |
|
PMID: 32318290 |
Sample: Serum |
M*****iSubmitted [ Sep 23 2022 ]
Asked: Can this kit detect the activity of Mn-SOD?
Reply
adminSubmitted [ Sep 23 2022 ]
Answered: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.
s************eSubmitted [ Mar 19 2019 ]
Asked: can we used these kits for microlab 300. Thank you
Reply
adminSubmitted [ Mar 19 2019 ]
Answered: I'm sorry this kit can't be used for microlab 300.
A******aSubmitted [ Jan 30 2019 ]
Asked: Kit no catalog E-BC-K022 can be used for what species? Rat, mice or human? Thank you
Reply
adminSubmitted [ Jan 30 2019 ]
Answered: Hi Agnestia, Thanks for your question. This kit Cat.# E-BC-K022 is an universal kit and can be used for rat, mice, human and other species. Please feel free to let us know if any question. Thank you.
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A******aSubmitted [ Jan 30 2019 ]
Asked: This kit can be used for what? rat, human, or mice? Thank you
Reply