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CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

  • Cat.No.:E-BC-K022-S

  • Detection instrument: Spectrophotometer(550 nm)

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Detection principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Performance characteristics

Synonyms CuZn-SOD,T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 2.03 U/mL
Detection range 2.03-155 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 4%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume added to the reaction (μL)

Mouse serum

3-5

20-30

Human serum

1

30-40

Rat serum

3-5

20-30

Rat plasma

1-2

20-30

Human hydrothorax

1

30-50

Human urine

1

30-50

10% Mouse liver tissue homogenate

50-80

20-30

10% Mouse brain tissue homogenate

8-12

20-30

10% Mouse kidney tissue homogenate

10-20

20-30

HepG2 cells (3.27 mgprot/mL)

5-10

20-30

Note: The diluent is normal saline (0.9% NaCl).

Citations

  1. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    Sample: Liver
  2. Journal of Nutrition and Metabolism (2020)
    Digestive Fermentation, Antioxidant Status, and Haemato-Biochemical Indices of Growing Rabbits Fed on Diets Supplemented with Ziziphus spina-christi Leaf

    DOI: 10.1155/2020/9046862

    PMID: 32318290

    Sample: Serum
  3. TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
    Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

    DOI: 10.1177/0748233720937199

    Sample: Plasma
  4. ANDROLOGIA (2020) IF: 1.951
    Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

    DOI: 10.1111/and.13825

    PMID: 32926461

    Sample: Cell Lysate
  5. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    Sample: Plasma,Tissue Homogenate
  6. JOURNAL OF PHARMACY AND PHARMACOLOGY (2020) IF: 2.571
    By reducing oxidative stress, naringenin mitigates hyperglycaemia‐induced upregulation of hepatic nuclear factor erythroid 2‐related factor 2 protein

    DOI: 10.1111/jphp.13319

    Sample: Cell Lysate
  7. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    HPLC-DAD analysis of Hyssopus Cuspidatus Boriss extract and mensuration of its antioxygenation property

    DOI: 10.1186/s12906-020-03016-0

    PMID: 32689984

    Sample: Plant Fluid
  8. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    Sample: Cell
  9. BRAIN RESEARCH BULLETIN (2022) IF: 3.715
    Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

    DOI: 10.1016/j.brainresbull.2022.09.015

    PMID: 36152772

    Sample: Brain Tissue
  10. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    Sample: Myocardium
  11. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue Homogenate
  12. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2978340

    PMID: 32655765

    Sample: Plasma,Urine,Tissue Homogenate
  13. Fermentation-Basel (2022) IF: 5.123
    Antidiabetic and Hypolipidemic Efficiency of Lactobacillus plantarum Fermented Oat (Avena sativa) Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/fermentation8060267

    Sample: Serum
  14. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    Sample: Serum
  15. Antioxidants (2022) IF: 6.313
    Evaluating the Nutritional and Immune Potentiating Characteristics of Unfermented and Fermented Turmeric Camel Milk in Cyclophosphamide-Induced Immunosuppression in Rats

    DOI: 10.3390/antiox11040792

    PMID: 35453477

    Sample: Blood
  16. Antioxidants (2022) IF: 6.313
    Antioxidative, Antidiabetic, and Hypolipidemic Properties of Probiotic-Enriched Fermented Camel Milk Combined with Salvia officinalis Leaves Hydroalcoholic Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/antiox11040668

    PMID: 35453353

    Sample: Serum
  17. Antioxidants (2022) IF: 6.313
    Ameliorative and Antioxidative Potential of Lactobacillus plantarum-Fermented Oat (Avena sativa) and Fermented Oat Supplemented with Sidr Honey against Streptozotocin-Induced Type 2 Diabetes in Rats

    DOI: 10.3390/antiox11061122

    Sample: Serum
  18. PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
    The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

    DOI: 10.1111/pce.14263

    PMID: 35040151

    Sample: Leaf
  19. Antioxidants (2022) IF: 7.675
    Phenolics and Volatile Compounds of Fennel (Foeniculum vulgare) Seeds and Their Sprouts Prevent Oxidative DNA Damage and Ameliorates CCl4-Induced Hepatotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox11122318

    Sample: Serum
  20. Antioxidants (2023) IF: 7.675
    Nephroprotective Effect of Fennel (Foeniculum vulgare) Seeds and Their Sprouts on CCl4-Induced Nephrotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox12020325

    Sample: Serum,Kidney Tissue
  21. Antioxidants (2022) IF: 7.675
    Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma

    DOI: 10.3390/antiox11112264

    Sample: Plasma
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Reviews/Q&A

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Q

M*****iSubmitted [ Sep 23 2022 ]

Asked: Can this kit detect the activity of Mn-SOD?

Reply

A

adminSubmitted [ Sep 23 2022 ]

Answered: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.

Q

s************eSubmitted [ Mar 19 2019 ]

Asked: can we used these kits for microlab 300. Thank you

Reply

A

adminSubmitted [ Mar 19 2019 ]

Answered: I'm sorry this kit can't be used for microlab 300.

Q

A******aSubmitted [ Jan 30 2019 ]

Asked: Kit no catalog E-BC-K022 can be used for what species? Rat, mice or human? Thank you

Reply

A

adminSubmitted [ Jan 30 2019 ]

Answered: Hi Agnestia, Thanks for your question. This kit Cat.# E-BC-K022 is an universal kit and can be used for rat, mice, human and other species. Please feel free to let us know if any question. Thank you.

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Asked: This kit can be used for what? rat, human, or mice? Thank you

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