EIF5A Polyclonal Antibody
- +1
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human;Mouse
- Applications: WB;IHC;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:HeLa,293T,Raji,22Rv1,HepG2,U-251MG,Mouse testis Verified Samples in IHC:Human kidney Verified Samples in IF:U2OS |
Dilution |
WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human EIF5A (NP_001137234.1). |
Abbre | EIF5A |
Synonyms | EIF5A;EIF-5A;EIF5A1;eIF5AI |
Swissprot | |
Calculated MW | 16kDa/20kDa |
Observed MW |
17kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | EIF5A (Eukaryotic Translation Initiation Factor 5A) is a Protein Coding gene. Diseases associated with EIF5A include Human Immunodeficiency Virus Type 1 and Retinitis Pigmentosa 14. Among its related pathways are eIF5A regulation in response to inhibition of the nuclear export system and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include protein N-terminus binding. An important paralog of this gene is EIF5AL1. |