Alternate Names	Glycophorin-A/B,GYPA/B,MN sialoglycoprotein,SS-active sialoglycoprotein,PAS-2/3,Sialoglycoprotein alpha/delta,CD235a/b
Swissprot	P02724,P06028
References	1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York. 2. Bain BJ. Leukemia diagnosis: A guide to the FAB classification. 1990.

Product Details

Form	Liquid
Clone No.	HIR2(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2b, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD235 Antibody[HIR2]	25μg, 100μg	Check More
Biotin Anti-Human CD235 Antibody[HIR2]	25μg, 100μg	Check More
FITC Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD235 Antibody[HIR2]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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