Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 488 Anti-Human CD235 Antibody[HIR2]
Cat.No.:E-AB-F1080L
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Alternate Names | Glycophorin-A/B,GYPA/B,MN sialoglycoprotein,SS-active sialoglycoprotein,PAS-2/3,Sialoglycoprotein alpha/delta,CD235a/b |
Swissprot | P02724,P06028 |
References |
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
2. Bain BJ. Leukemia diagnosis: A guide to the FAB classification. 1990. |
Form | Liquid |
Clone No. | HIR2(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2b, κ |
Isotype Control | Elab Fluor® 488 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812L] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 488 |
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Human CD235 Antibody[HIR2] | 25μg, 100μg | Check More |
Biotin Anti-Human CD235 Antibody[HIR2] | 25μg, 100μg | Check More |
FITC Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD235 Antibody[HIR2] | 20Tests, 100Tests, 100Tests×2 | Check More |
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