Alternate Names	Low affinity immunoglobulin gamma Fc region receptor II-b,FCGR2B,IgG Fc receptor II-b,CDw32,Fc-gamma RII-b,Fc-gamma-RIIb,FcRII-b,CD32,FCG2, IGFR2
Swissprot	P31994
References	1. Stuart S, et al. 1989. EMBO J. 8:3657. 2. Huang Y, et al. 1999. Scand. J. Immunol. 49:177. 3. Hisaka H, et al. 1999. Pathobiology 67:92.

Product Details

Form	Liquid
Clone No.	IV-3(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2b, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD32 Antibody[IV-3]	25μg, 100μg	Check More
Biotin Anti-Human CD32 Antibody[IV-3]	25μg, 100μg	Check More
FITC Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD32 Antibody[IV-3]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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