Alternate Names	Lymphocyte function-associated antigen 3,CD58,Ag3,Surface glycoprotein LFA-3,LFA3
Swissprot	P19256
References	1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223. 2. Dustin ML, et al. 1987. Nature 329:846. 3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861. 4. Sanders ME, et al. 1988. J. Immunol. 140:1401.

Product Details

Form	Liquid
Clone No.	TS2/9.1(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068A	25μg, 100μg	Check More
Biotin Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068B	25μg, 100μg	Check More
PE Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068D	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068E	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068G	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD58 Antibody[TS2/9.1] E-AB-F1068H	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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