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Elab Fluor^® 488 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]

Uniprot : P27512

Cat.No.:E-AB-F1028L
Applications: FCM
Isotype: Rat IgG2a, κ
Host: Rat

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Antigen Details

Alternate Names	Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5
Swissprot	P27512
References	1. Hasbold J, Johnson-Leger C, Atkins CJ, Clark EA, Klaus GG. Eur J Immunol. 1994; 24(8):1835-1842. 2. Hasbold J, Klaus GG. Eur J Immunol. 1994; 24(1):152-157. 3. Klaus GG, Holman M, Hasbold J. Eur J Immunol. 1994; 24(11):2714-2719. 4. Noelle RJ, Ledbetter JA, Aruffo A. Immunol Today. 1992; 13(11):431-433. 5. Parry SL, Hasbold J, Holman M, Klaus GG. J Immunol. 1994; 152(6):2821-2829.

Product Details

Form	Liquid
Clone No.	FGK4.5/FGK45(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	Elab Fluor^® 488 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832L]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
Biotin Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
FITC Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PE Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
APC Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]	25μg, 100μg	Check More

Citations

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