Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 647 Anti-Mouse CD11a Antibody[FD441.8]
Cat.No.:E-AB-F1033UM
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Alternate Names | Integrin alpha-L,Itgal,CD11 antigen-like family member A,LFA-1A,Lymphocyte antigen 15,Ly-15,CD11a |
Swissprot | P24063 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301. 3. Lub M, et al. 1995. Immunol. Today 16:479. |
Form | Liquid |
Clone No. | FD441.8(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Elab Fluor® 647 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843M] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 647 |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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