Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]
Cat.No.:E-AB-F1132M
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Alternate Names | B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1 |
Swissprot | Q9EP73 |
References |
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1039. |
Form | Liquid |
Clone No. | 10F.9G2(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Elab Fluor® 647 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842M] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 647 |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50μg, 500μg, 1mg | Check More |
Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Biotin Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Elab Fluor® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
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