Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 647 Anti-Mouse CD62L Antibody[Mel14]
Cat.No.:E-AB-F1011M
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22 |
Swissprot | P18337 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259. |
Form | Liquid |
Clone No. | Mel14(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | Elab Fluor® 647 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832M] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 647 |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). | |
|
How to select the appropriate detection channel through the spectrogram?
CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and nave T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte 'rolling' on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish nave, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!