EpCAM Polyclonal Antibody (E-AB-40300)
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For research use only.
| Verified Samples |
Verified Samples in WB: Rat heart, Mouse liver Verified Samples in IHC: Human lung cancer, Human colon cancer, Mouse colon |
| Dilution | WB 1:500-1:1000, IHC 300-1:700 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human SDHA protein expressed by E.coli |
| Abbre | EpCAM |
| Synonyms | 17 1A, 323/A3, AUA1, Adenocarcinoma associated antigen, Adenocarcinoma-associated antigen, Antigen identified by monoclonal AUA1, CD326, CD326 antigen, CO 17A, CO17 , Cell surface glycoprotein Trop 1, Cell surface glycoprotein Trop 2, Cell surface glycoprotein Trop-1 |
| Swissprot | |
| Calculated MW | 35,40 kDa |
| Observed MW |
35 ,40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell junction, Cell membrane, Membrane, Tight junction. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Epithelial cell adhesion molecule (EpCAM,CD326) is a type I transmembrane glycoprotein that functions as a homophilic,epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion,EpCAM is also involved in cellular signaling,cell migration,proliferation,and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas,and has become a therapeutic target. |
Other Clones
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Unconjugated
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