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FITC Anti-Human CD235 Antibody[HIR2]

Uniprot : P02724,P06028
  • Cat.No.:E-AB-F1080C

  • Applications: FCM
  • Isotype: Mouse IgG2b, κ
  • Host: Mouse

To Purchase E-AB-F1080C

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  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $64
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Antigen Details

Alternate Names Glycophorin-A/B,GYPA/B,MN sialoglycoprotein,SS-active sialoglycoprotein,PAS-2/3,Sialoglycoprotein alpha/delta,CD235a/b
Swissprot P02724,P06028
References
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
2. Bain BJ. Leukemia diagnosis: A guide to the FAB classification. 1990.

Product Details

Form Liquid
Clone No. HIR2
Host Mouse
Isotype Mouse IgG2b, κ
Isotype Control FITC Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812C]
Reactivity Human
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
Purified Anti-Human CD235 Antibody[HIR2] 25μg, 100μg Check More
Biotin Anti-Human CD235 Antibody[HIR2] 25μg, 100μg Check More
PE Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD235 Antibody[HIR2] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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