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FITC Anti-Mouse CD73 Antibody[TY/23]

  • Cat.No.:E-AB-F1089C

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1089C

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Antigen Details

Alternate Names 5'-nucleotidase,Nt5e,5'-NT,Ecto-5'-nucleotidase,CD73,Nt5, Nte,Nt5e
Swissprot Q61503
References
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Product Details

Form Liquid
Clone No. TY/23(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832C]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

  1. FASEB JOURNAL (2022) IF: 5.834
    The fates of different types of adipose tissue after transplantation in mice

    DOI: 10.1096/fj.202200408R

    PMID: 36004579

    Sample: P3 Cell

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