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FITC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]

  • Cat.No.:E-AB-F1094UC

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F1094UC

Size:
  • 25μg
  • 100μg
Price: $30
Qty:

Antigen Details

Alternate Names Thy-1.2 membrane glycoprotein,Thy1.2,Thy-1.2 antigen,CD90.2,Thy-1.2
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Product Details

Form Liquid
Clone No. 30H12
Host Rat
Isotype Rat IgG2b, κ
Isotype Control FITC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843C]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
Biotin Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
FITC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
APC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
PerCP Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12] 25μg, 100μg Check More

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