β-galactosidase (β-GAL) Activity Assay Kit (E-BC-K631-M)
For research use only.
| Detection Principle | β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm. |
| Synonyms | β-GAL |
| Sample Type | Cell,Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (390-410 nm, optimum wavelength: 400 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | 2 mmol/L hydrochloric acid |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 16.19 U/L |
| Detection Range | 16.19-1200 U/L |
| Precision | inter-assay CV: 6.0% | intra-assay CV: 3.0% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 3-5 |
| 10% Rat liver tissue homogenate | 1-3 |
| 10% Rat heart tissue homogenate | 1 |
| Human serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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