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Glutamate Dehydrogenase (GDH) Activity Assay Kit

Cat.No.:E-BC-K759-M
Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

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96T

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Detection principle

GDH catalyzes the dehydrogenation of glutamate, meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of GDH can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	GDH
Sample type	serum (plasma), urine, animal tissue
Sensitivity	0.54 U /L
Detection range	0.54-25.0 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	35 min
Precision	Average inter-assay CV: 4%Average intra-assay CV: 3%
Other instruments required	Incubator(37℃), centrifuge
Other reagents required	Double distilled water
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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