Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K143

      Size:
      • R1:37mL×2,R2:10mL×2
      Qty:
      - +
      Price: $660
      Can detect 98 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Biochemistry analyzer(340 nm), Spectrophotometer(340 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

      Performance characteristics

      Synonyms Hcy
      Sample type serum
      Detection range 0-50 μmol/L
      Storage 2-8℃
      Valid period 12 months

      Citations

      1. Attenuation of Hyperhomocysteinemia Induced Vascular Dementia by Sodium Orthovanadate Perhaps via PTP1B: Pertinent Downstream Outcomes
        IF: 2.77
        Journal: Behavioural Brain Research(2019)
        DOI: 10.1016/j.bbr.2019.01.039
        PMID: 30721761
        Products: E-BC-K143 
        Sample type: Tissue homogenate
        Reactivity: Mouse
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      MSDS for ELISA ISO 9001

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