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Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

Cat.No.:E-BC-K143
Detection instrument: Biochemistry analyzer(340 nm), Spectrophotometer(340 nm)

Detection method: Colorimetric method

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Detection principle

Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

Performance characteristics

Synonyms	Hcy
Sample type	Serum
Detection range	0-50 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Food & Function (2022) IF: 5.396
L-Methionine supplementation attenuates high-fat fructose diet-induced non-alcoholic steatohepatitis by modulating lipid metabolism, fibrosis and inflammation in rats

DOI: 10.1039/D1FO03403K

Sample: plasma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Vitamin B Mitigates Thoracic Aortic Dilation in Marfan Syndrome Mice by Restoring the Canonical TGF-β Pathway

DOI: 10.3390/ijms222111737

PMID: 34769168
Sample: plasma
Biomolecules (2022) IF: 4.879
Hyperhomocysteinemia Increases Cortical Excitability and Aggravates Mechanical Hyperalgesia and Anxiety in a Nitroglycerine-Induced Migraine Model in Rats

DOI: 10.3390/biom12050735

PMID: 35625662
Sample: plasma
Current Medical Science (2021) IF: 2.135
Ligustrazine Attenuates Hyperhomocysteinemia-induced Alzheimer-like Pathologies in Rats

DOI: 10.1007/s11596-021-2379-1

PMID: 34169425
Sample: plasma
JOURNAL OF CLINICAL LABORATORY ANALYSIS (2021) IF: 2.352
Exploring potential serum levels of Homocysteine, interleukin-1 beta, and apolipoprotein B 48 as new biomarkers for patients with ischemic stroke

DOI: 10.1002/jcla.23996

PMID: 34492129
Sample: serum
AMERICAN JOURNAL OF THE MEDICAL SCIENCES (2021) IF: 2.378
MTHFR C677T Polymorphism and Serum Homocysteine Level as Risk Factors of Coronary Heart Disease in Patients with Androgenetic Alopecia: A Case Control Study

DOI: 10.1016/j.amjms.2021.06.013

Sample: blood
BEHAVIOURAL BRAIN RESEARCH (2019) IF: 2.77
Attenuation of hyperhomocysteinemia induced vascular dementia by sodium orthovanadate perhaps via PTP1B: Pertinent downstream outcomes

DOI: 10.1016/j.bbr.2019.01.039

PMID: 30721761
Sample: Tissue homogenate
Journal of Stroke & Cerebrovascular Diseases (2020) IF: 1.787
Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage

DOI: 10.1016/j.jstrokecerebrovasdis.2020.105537

PMID: 33338706
Sample: plasma
Journal of Blood Medicine (2019)
Serum homocysteine and disease severity in sickle cell anemia patients in Lagos

DOI: 10.2147/JBM.S198316

Sample: blood
Neurochemical Journal (2022) IF: 0.448
Levels of Protein Carbonylation and Activity of Proteases in the Brain of Newborn Rats with Prenatal Hyperhomocysteinemia

DOI: 10.1134/S181971242203014X

Sample: plasma
African Health Sciences (2020) IF: 0.6899
Relationship between plasma homocysteine and blood pressure in hypertensive Northern-Nigerians

DOI: 10.4314/ahs.v20i1.38

Sample: plasma

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