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Human IFN-β(Interferon Beta) ELISA Kit

  • Cat.No.:E-EL-H0085

  • Reactivity: Human

To Purchase E-EL-H0085

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 105.20 289.60 951.90 107.40 261.00 1001.00
Standard deviation 5.90 14.20 40.00 5.40 11.70 54.10
CV (%) 5.61 4.90 4.20 5.03 4.48 5.40

Recovery

The recovery of Human IFN-β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 93
EDTA plasma (n=8) 92-106 98
Cell culture media (n=8) 86-98 93

Linearity

Samples were spiked with high concentrations of Human IFN-β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01574
SynonymsIFNB1, IFB, IFF, IFNB
Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International Journal of Molecular Sciences (2021) IF: 4.556
    Microengineered Bioartificial Liver Chip for Drug Toxicity Screening.

    DOI: 10.3390/ijms22094668

    PMID: 33925089

    Sample: Cell lysate
  2. Cell Reports (2019) IF: 7.815
    The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization

    DOI: 10.1016/j.celrep.2019.01.030

    PMID: 30699354

    Sample: Cell culture supernatant
  3. Journal of virology (2020) IF: 4.501
    Oral vitamin-A-coupled valsartan nanomedicine: High hepatic stellate cell receptors accessibility and prolonged enterohepatic residence.

    DOI: 10.1128/JVI.02001-20

    PMID: 33361428

    Sample: Cell culture supernatant
  4. Cell death & disease (2020) IF: 6.304
    Annexin-A1 promotes RIG-I-dependent signaling and apoptosis via regulation of the IRF3-IFNAR-STAT1-IFIT1 pathway in A549 lung epithelial cells

    DOI: 10.1038/s41419-020-2625-7

    PMID: 32541772

    Sample: Cell culture supernatant
  5. Cell Death & Disease (2020) IF: 6.304
    Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

    DOI: 10.1038/s41419-020-2665-z

    PMID: 32532953

    Sample: Cell culture supernatant
  6. Frontiers in Immunology (2017) IF: 6.429
    Influenza A Virus Pa Antagonizes Interferon-β By Interacting With Interferon Regulatory Factor 3.

    DOI: 10.3389/fimmu.2017.01051

    PMID: 28955326

    Sample: Cell culture supernatant
  7. Frontiers in Immunology (2017) IF: 6.429
    The C-Terminal Effector Domain Of Non-Structural Protein 1 Of Influenza A Virus Blocks IFN-β Production By Targeting TNF Receptor-Associated Factor 3.

    DOI: 10.3389/fimmu.2017.00779

    PMID: 28717359

    Sample: Cell culture supernatant
  8. Clinical & Experimental Allergy (2020) IF: 4.217
    Relationship between immune parameters during a severe exacerbation in allergic asthmatic children and asthma outcomes in the following year

    DOI: 10.1111/cea.13570

    PMID: 31955476

    Sample: Plasma
  9. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019) IF: 4.328
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    Sample: Serum
  10. Journal of Virology (2017) IF: 4.663
    MMP-9 Facilitates Hepatitis B Virus Replication Through Binding With IFNAR1 To Repress IFN/JAK/STAT Signaling.

    DOI: 10.1128/JVI.01824-16

    PMID: 28122987

    Sample: Cell culture supernatant
  11. Frontiers in Microbiology (2017) IF: 4.076
    miR-194 Inhibits Innate Antiviral Immunity By Targeting FGF2 In Influenza H1N1 Virus Infection.

    DOI: 10.3389/fmicb.2017.02187

    PMID: 29163456

    Sample: Cell culture supernatant,Bronchoalveolar Lavage Fl
  12. Journal of Virology (2017) IF: 4.663
    Matrix Metalloproteinase 9 Facilitates Hepatitis B Virus Replication Through Binding With IFNAR1 To Repress IFN/JAK/STAT Signaling.

    DOI: 10.1128/JVI.01824-16

    PMID: 28122987

    Sample: Cell culture supernatant
  13. Journal of Immunology (2016) IF: 4.856
    A Novel Function Of F-Box Protein FBXO17 In Negative Regulation Of Type I IFN Signaling By Recruiting PP2A For IFN Regulatory Factor 3 Deactivation.

    DOI: 10.4049/jimmunol.1601009

    PMID: 27956528

    Sample: Cell culture supernatant
  14. Scientific Reports (2020) IF: 3.998
    Cellular uptake of extracellular nucleosomes induces innate immune responses by binding and activating cGMP-AMP synthase (cGAS)

    DOI: 10.1038/s41598-020-72393-w

    PMID: 32958884

    Sample: Cell culture supernatant
  15. Scientific reports (2019) IF: 3.998
    Messenger RNA translation enhancement by immune evasion proteins: a comparative study between EKB (vaccinia virus) and NS1 (influenza A virus)

    DOI: 10.1038/s41598-019-48559-6

    PMID: 31427778

    Sample: Cell culture supernatant
  16. Innate Immunity (2020) IF: 2.298
    Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways

    DOI: 10.1177/1753425919877680

    PMID: 31550955

    Sample: Cell culture supernatant
  17. Immunopharmacology and Immunotoxicology (2020) IF: 2.352
    Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells

    DOI: 10.1080/08923973.2020.1725040

    PMID: 32048561

    Sample: Cell culture supernatant
  18. International Immunopharmacology (2018) IF: 2.956
    Inhibition Of Curcumin On Influenza A Virus Infection And Influenzal Pneumonia Via Oxidative Stress, TLR2/4, p38/JNK MAPK And NF-κB Pathways.

    DOI: 10.1016/j.intimp.2017.11.009

    PMID: 29153953

    Sample: Cell culture supernatant
  19. Archives of Virology (2014) IF: 2.058
    Hepatitis C Virus Infection Decreases The Expression Of Toll-Like Receptors 3 And 7 Via Upregulation Of Mir-758.

    DOI: 10.1007/s00705-014-2167-3

    PMID: 25008898

    Sample: Cell culture supernatant
  20. International Journal of Molecular Sciences (2021) IF: 4.556
    Broadly Antiviral Activities of TAP1 through Activating the TBK1-IRF3-Mediated Type I Interferon Production

    DOI: 10.3390/ijms22094668

    PMID: 33925089

    Sample: Cell lysate
  21. Journal of virology (2020) IF: 4.501
    Protein tyrosine phosphatase SHP2 suppresses host innate immunity against influenza A virus through regulating EGFR-mediated signaling

    DOI: 10.1128/JVI.02001-20

    PMID: 33361428

    Sample: Cell culture supernatant
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