IL36A Polyclonal Antibody (E-AB-18108)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse liver, Human tonsil Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human IL36A |
| Abbre | IL36A |
| Synonyms | FIL, IL1F, Interleukin-1 Family Member, IL-1F, IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, FIL1 Epsilon, IL-1 Epsilon, Interleukin-1 Epsilon, Interleukin-1 Family Member 6, Interleukin-36 Alpha, IL1E, IL 1 epsilon, IL 1F6, IL 1H1, IL1RP2, Interleukin 1 epsilon, Interleukin 1 family member 6, Interleukin 1 family member 6 (epsilon), Interleukin 36 alpha, MGC129552, MGC129553, MGC151479, MGC151481, OTTMUSP00000012798, RP23-176J12.4 |
| Swissprot | |
| Calculated MW | 18 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted. |
| Concentration | 0.78 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Immunology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | IL36A (Interleukin 36, Alpha) is a Protein Coding gene. Diseases associated with IL36A include Psoriasis. Among its related pathways are Innate Immune System and Toll-Like receptor Signaling Pathways. GO annotations related to this gene include cytokine activity and interleukin-1 receptor binding. An important paralog of this gene is IL36G.The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. |
Other Clones
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Unconjugated
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