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KLF6 Polyclonal Antibody

Cat:E-AB-52129
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:293T
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human KLF6
Abbre KLF6
Synonyms B cell derived protein 1;B cell-derived 1;B-cell-derived protein 1;BCD1;CBA1;COPEB;Core promoter element-binding protein;CPBP;GBF;GC rich binding factor;GC rich sites binding factor GBF;GC-rich sites-binding factor GBF;Klf6;KLF6;Krueppel-like factor 6;Krueppel-like factor 6 isoform C;Kruppel like zinc finger protein Zf9;PAC1;Proto-oncogene BCD1;Protooncogene B cell derived 1;ST12;Suppression of tumorigenicity 12 (prostate);Suppressor of tumorigenicity 12 protein;Transcription factor Zf9;Zf9
Swissprot
Calculated MW 32 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Stem cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.