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Laminin alpha5 Polyclonal Antibody

Cat:E-AB-31903 Citations (3)
Manual MSDS

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Size:
200μL 120μL 60μL 20μL
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  • Host: Rabbit
  • Reactivity: Human;Mouse
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:RAW264.7,HepG2,A549
Dilution

WB 1:500-1:2000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from the Internal region of human Laminin α-5
Abbre Laminin α-5
Synonyms LAMA5;KIAA0533;KIAA1907;Laminin subunit alpha-5;Laminin-10 subunit alpha;Laminin-11 subunit alpha;Laminin-15 subunit alpha
Swissprot
Calculated MW 400kDa
Observed MW 400kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Extracellular region or secreted,basal lamina,basement membrane,extracellular exosome,extracellular matrix,extracellular region,extracellular space,laminin-10 complex,laminin-11 complex,laminin-5 complex,synaptic cleft,Nucleus
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.