Lipase (LPS) Activity Assay Kit (E-BC-K786-M)
For research use only.
| Detection Principle | Lipase can catalyze the substrate to produce sulfhydryl compounds, which react with DTNB to generate TNB. TNB has the maximum absorption at 412 nm. The activity of LPS in the sample can be calculated by measuring the change of absorbance per unit time. |
| Synonyms | LPS |
| Sample Type | Serum,Plasma,Animal tissue,Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-420 nm, optimum wavelength: 412 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Ethanol absolute (99.5%) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-9.41 U/L |
| Precision | inter-assay CV: 0.06 | intra-assay CV: 0.03 |
| Sample Volume | 20 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 6-10 |
| 10% Rat brain tissue homogenate | 2-6 |
| 10% Rat kidney tissue homogenate | 3-6 |
| 10% Rat liver tissue homogenate | 5-10 |
| 10% Rat heart tissue homogenate | 2-5 |
| 10% Rat lung tissue homogenate | 3-6 |
| 10% Rat spleen tissue homogenate | 2-5 |
| 10% Mouse lung tissue homogenate | 3-6 |
| Human serum | 7-10 |
| Human plasma | 2-6 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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