Lipase can catalyze the substrate to produce sulfhydryl compounds, which react with DTNB to generate TNB. TNB has the maximum absorption at 412nm. The activity of LPS in the sample can be calculated by measuring the change of absorbance per unit time.

Performance characteristics

Synonyms	LPS
Sample type	serum, plasma, animal tissue, cell samples
Sensitivity	0.03 U/L
Detection range	0.03-9.41 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3%
Other instruments required	Incubator, Centrifuge, Microplate reader
Other reagents required	Ethanol absolute (99.5%)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
Dendrobium officinale polysaccharide attenuates cognitive impairment in circadian rhythm disruption mice model by modulating gut microbiota

DOI: 10.1016/j.ijbiomac.2022.07.090

PMID: 35853505
Sample: serum
Food & Function (2022) IF: 6.317
Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

DOI: 10.1039/D2FO00477A

Sample: serum
Journal of Functional Foods (2021) IF: 4.451
Effect of κ-carrageenan on glucolipid metabolism and gut microbiota in high-fat diet-fed mice

DOI: 10.1016/j.jff.2021.104707

Sample: serum
BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738
Role of Combination Treatment of Aspirin and Zinc in DMH-DSS-induced Colon Inflammation, Oxidative Stress and Tumour Progression in Male BALB/c Mice

DOI: 10.1007/s12011-022-03241-3

Sample: plasma

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Reviews/Q&A

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Reviews
Q&A

M******** Submitted [ Jun 13 2023 ]

Asked: Is the lipase kit can be used for the analysis of lipase in pharmaceutical products ??

adminSubmitted [ Jun 13 2023 ]

Answered: Dear May, thanks for your support! Yes, the lipase kit can be used for the analysis of lipase in pharmaceutical products.

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Cat. No	E-BC-K786-M
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