Lipase can catalyze the substrate to produce sulfhydryl compounds, which react with DTNB to generate TNB. TNB has the maximum absorption at 412nm. The activity of LPS in the sample can be calculated by measuring the change of absorbance per unit time.

Performance characteristics

Synonyms	LPS
Sample type	serum, plasma, animal tissue, cell samples
Sensitivity	0.03 U/L
Detection range	0.03-9.41 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3%
Other instruments required	Incubator, Centrifuge, Microplate reader
Other reagents required	Ethanol absolute (99.5%)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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