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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

  • Cat.No.:E-BC-K025-S

  • Detection instrument: Spectrophotometer(532 nm)

To Purchase E-BC-K025-S

Size:
  • 100Assays
  • 50Assays
Price: $220
Qty:

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Serum,plasma,animal tissue
Sensitivity 0.38 nmol/mL
Detection range 0.38-133.33 nmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 90 min
Precision Average inter-assay CV: 8%Average intra-assay CV: 4.900%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 6 months

Images

The level of MDA was significantly increased (*P<0.005) in cells treating with the nanoreactor.

XX Yang et al investigates the function of a nanoreactor that can induce ferroptosis of tumor cells by releasing DHA and iron. Malondialdehyde (MDA) of Lewis lung cancer cells was determined using MDA colorimetric assay kit (E-BC-K025-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Mouse plasma

1

10% Rat heart tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Behavioural Brain Research (2020) IF: 2.977
    Neuroprotective action of agmatine in rotenone-induced model of Parkinson's disease: Role of BDNF/cREB and ERK pathway

    DOI: 10.1016/j.bbr.2020.112692

    PMID: 32479847

    Sample: Tissue homogenate
  2. Naunyn-Schmiedeberg's Archives of Pharmacology (2019) IF: 2.058
    Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice

    DOI: 10.1007/s00210-019-01626-1

    PMID: 30759263

    Sample: Serum,Tissue homegenate
  3. Molecular Biology Reports (2019) IF: 2.107
    Efficacy, toxicity study and antioxidant properties of plantaricin E and F recombinants against enteropathogenic Escherichia coli K1.1 (EPEC K1.1)

    DOI: 10.1007/s11033-019-05096-9

    PMID: 31583564

    Sample: Tissue homogenate
  4. Annals of Tropical Medicine & Public Health (2020)
    A study for Malondialdehyde and lipid profile levels in ischemic stroke patients with and without diabetes mellitus

    DOI: 10.36295/ASRO.2020.23115

    Sample: Serum
  5. Latin American Journal of Pharmacy (2019) IF: 0.24
    The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
    Sample: Serum,Tissue homogenate
  6. Jurnal Farmasi (2019)
    STUDI PRELIMINARI TENTANG PENGARUH D-GALAKTOSA DALAM MENGINDUKSI STRES OKSIDATIF PADA MENCIT JANTAN GALUR OUTBRED FK USU

    DOI: 10.35451/jfm.v2i1.191

    Sample: Serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

F***********dSubmitted [ Sep 14 2019 ]

  • Application:Biochemical
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Verified Customer

V*****tSubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The reaction time of this biochemical kit is a little long, and the acetic acid reagent needs to be prepared by yourself. The sample volume needs to be determined by yourself, but for some samples, the reference sample volume is provided in the instructions, which is great.Moreover, the method for calculating the results of this kit is simple and accurate
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Q

M*****aSubmitted [ Aug 09 2022 ]

Asked: Is the kit suitable for cell sample?

Reply

A

adminSubmitted [ Aug 09 2022 ]

Answered: 

A

adminSubmitted [ Aug 09 2022 ]

Answered: No, if you want to detect cell sample, please consider the cat#E-BC-K028-M.

Q

M*****aSubmitted [ Aug 02 2022 ]

Asked: MDA assay test, is it possible to do in ordinary lab human blood or urine?

Reply

A

adminSubmitted [ Aug 02 2022 ]

Answered: Sorry, it can test blood, but not urine.

Q

j**aSubmitted [ Jul 04 2022 ]

Asked: I have used an MDA kit with code E-BC-K025-S and a CuZn-SOD/Mn-SOD kit. I want to ask where I can get the normal range of results that I get from the human serum test

Reply

A

adminSubmitted [ Jul 04 2022 ]

Answered: The following data are some of the results of our verification, you can also find some literatures to get the normal range ya: MDA---E-BC-K025-M---11.66 μmol/L CuZn-SOD/Mn-SOD----E-BC-K022-S---72 U/mL

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