Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K025-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $220
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(532 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

      Performance characteristics

      Synonyms MDA
      Sample type Serum,plasma,animal tissue
      Sensitivity 0.38 nmol/mL
      Detection range 0.38-133.33 nmol/mL
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 90 min
      Precision Average inter-assay CV: 8.0%Average intra-assay CV: 4.9%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      Human plasma

      1

      Rat serum

      1

      Rat plasma

      1

      Mouse serum

      1

      Mouse plasma

      1

      10% Rat heart tissue homogenate

      1

      10% Rat liver tissue homogenate

      1

      10% Rat spleen tissue homogenate

      1

      10% Rat lung tissue homogenate

      1

      10% Rat kidney tissue homogenate

      1

      10% Rat brain tissue homogenate

      1

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)

      Citations

      1. Neuroprotective action of agmatine in rotenone-induced model of Parkinson's disease: Role of BDNF/cREB and ERK pathway
        IF: 2.977
        Journal: Behavioural Brain Research(2020)
        DOI: 10.1016/j.bbr.2020.112692
        PMID: 32479847
        Products: E-AB-70077 E-BC-K025-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice
        IF: 2.058
        Journal: Naunyn-Schmiedeberg's Archives of Pharmacology(2019)
        DOI: 10.1007/s00210-019-01626-1
        PMID: 30759263
        Products: E-BC-K025-S E-BC-K035-S E-BC-K019-S E-BC-K031-S 
        Sample type: Serum,Tissue homegenate
        Reactivity: Mouse
      3. Efficacy, toxicity study and antioxidant properties of plantaricin E and F recombinants against enteropathogenic Escherichia coli K1.1 (EPEC K1.1)
        IF: 2.107
        Journal: Molecular Biology Reports(2019)
        DOI: 10.1007/s11033-019-05096-9
        PMID: 31583564
        Products: E-BC-K025-S 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      4. A study for Malondialdehyde and lipid profile levels in ischemic stroke patients with and without diabetes mellitus
        Journal: Annals of Tropical Medicine & Public Health(2020)
        DOI: 10.36295/ASRO.2020.23115
        PMID: 99999999
        Products: E-BC-K025-S 
        Sample type: Serum
        Reactivity: Human
      5. The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
        IF: 0.24
        Journal: Latin American Journal of Pharmacy(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      6. STUDI PRELIMINARI TENTANG PENGARUH D-GALAKTOSA DALAM MENGINDUKSI STRES OKSIDATIF PADA MENCIT JANTAN GALUR OUTBRED FK USU
        Journal: Jurnal Farmasi(2019)
        DOI: 10.35451/jfm.v2i1.191
        PMID: 99999999
        Products: E-BC-K025-S 
        Sample type: Serum
        Reactivity: Mouse
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      Verified Customer

      F***********dSubmitted [ Sep 14 2019 ]

      • Application:Biochemical
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      Verified Customer

      V*****tSubmitted [ May 31 2019 ]

      • Application:Biochemical
      • Description:The reaction time of this biochemical kit is a little long, and the acetic acid reagent needs to be prepared by yourself. The sample volume needs to be determined by yourself, but for some samples, the reference sample volume is provided in the instructions, which is great.Moreover, the method for calculating the results of this kit is simple and accurate
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      MSDS for ELISA ISO 9001

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