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Mouse TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

Citations(22)Uniprot : P48787

  • Cat.No.:E-EL-M1203

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TNNI3/cTn-I were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TNNI3/cTn-I were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 25.50 62.70 175.10 24.70 60.50 162.00
Standard deviation 1.70 3.00 8.10 1.60 3.10 8.30
CV (%) 6.67 4.78 4.63 6.48 5.12 5.12

Recovery

The recovery of Mouse TNNI3/cTn-I spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 94
EDTA plasma (n=8) 86-101 93
Cell culture media (n=8) 92-107 99

Linearity

Samples were spiked with high concentrations of Mouse TNNI3/cTn-I and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P48787
SynonymsTNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1
Research AreaCancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Cardiovascular Diabetology (2022) IF: 9.9510
    Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis

    DOI: 10.1186/s12933-022-01532-6

    PMID: 35705980

    		 Sample: serum
  2. Frontiers in Cardiovascular Medicine (2022) IF: 6.05
    Sodium-Glucose Co-transporter-2 Inhibitor of Dapagliflozin Attenuates Myocardial Ischemia/Reperfusion Injury by Limiting NLRP3 Inflammasome Activation and Modulating Autophagy

    DOI: 10.3389/fcvm.2021.768214

    PMID: 35083298

    		 Sample: serum
  3. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Daming capsule protects against myocardial infarction by promoting mitophagy via the SIRT1/AMPK signaling pathway

    DOI: 10.1016/j.biopha.2022.113162

    PMID: 35676781

    		 Sample: serum
  4. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Curcumin modulates multiple cell death, matrix metalloproteinase activation and cardiac protein release in susceptible and resistant Plasmodium berghei-infected mice

    DOI: 10.1016/j.biopha.2021.112454

    PMID: 34894518

    		 Sample: serum
  5. Cancers (2022) IF: 6.639
    Attenuation of Muscle Damage, Structural Abnormalities, and Physical Activity in Respiratory and Limb Muscles following Treatment with Rucaparib in Lung Cancer Cachexia Mice

    DOI: 10.3390/cancers14122894

    PMID: 35740560

    		 Sample: plasma
  6. PHYTOMEDICINE (2022) IF: 6.656
    Shexiang Baoxin Pill attenuates myocardial ischemia/reperfusion injury by activating autophagy via modulating the ceRNA-Map3k8 pathway

    DOI: 10.1016/j.phymed.2022.154336

    PMID: 35849969

    		 Sample: serum,primary cardiomyocytes
  7. Stem Cell Research & Therapy (2021) IF: 6.832
    PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca2+ efflux

    DOI: 10.1186/s13287-021-02325-6
    Sample: Plasma
  8. Journal of Inflammation Research (2022) IF: 6.922
    Protective Effect of Crocin on Immune Checkpoint Inhibitors-Related Myocarditis Through Inhibiting NLRP3 Mediated Pyroptosis in Cardiomyocytes via NF-κB Pathway

    DOI: 10.2147/JIR.S348464
    Sample: serum
  9. Biomaterials Science (2019) IF: 5.251
    Targeted co-delivery of the aldose reductase inhibitor epalrestat and chemotherapeutic doxorubicin via a redox-sensitive prodrug approach promotes synergistic tumor suppression

    DOI: 10.1039/C9BM00221A
    Sample: serum
  10. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A

    DOI: 10.1111/jcmm.17160

    PMID: 35083842

    		 Sample: plasma
  11. PHYTOMEDICINE (2022) IF: 5.34
    Tetrahydrocurcumin improves lipopolysaccharide-induced myocardial dysfunction by inhibiting oxidative stress and inflammation via JNK/ERK signaling pathway regulation

    DOI: 10.1016/j.phymed.2022.154283

    PMID: 35779282

    		 Sample: serum
  12. Chinese Medicine (2021) IF: 5.455
    Full-length transcriptome analysis reveals the mechanism of acupuncture at PC6 improves cardiac function in myocardial ischemia model

    DOI: 10.1186/s13020-021-00465-8

    PMID: 34238326

    		 Sample: serum
  13. Journal of Translational Medicine (2021) IF: 5.531
    microRNA-193-3p attenuates myocardial injury of mice with sepsis via STAT3/HMGB1 axis

    DOI: 10.1186/s12967-021-03022-x

    PMID: 34503521

    		 Sample: serum
  14. Aging-US (2021) IF: 5.682
    Hypoxia-inducible factor 2-alpha-dependent induction of IL-6 protects the heart from ischemia/reperfusion injury

    DOI: 10.18632/aging.202276

    PMID: 33428604

    		 Sample: Plasma
  15. MOLECULES (2021) IF: 4.412
    Curcumin and Resveratrol Improve Muscle Function and Structure through Attenuation of Proteolytic Markers in Experimental Cancer-Induced Cachexia

    DOI: 10.3390/molecules26164904

    PMID: 34443492

    		 Sample: plasma
  16. Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
    ZYZ-803 Mitigates Endoplasmic Reticulum Stress-Related Necroptosis after Acute Myocardial Infarction through Downregulating the RIP3-CaMKII Signaling Pathway

    DOI: 10.1155/2019/6173685
    Sample: serum
  17. MOLECULAR PHARMACEUTICS (2022) IF: 4.939
    An Injectable In Situ Depot-Forming Lipidic Lyotropic Liquid Crystal System for Localized Intratumoral Drug Delivery

    DOI: 10.1021/acs.molpharmaceut.1c00782

    PMID: 35191706

    		 Sample: serum
  18. Scientific Reports (2022) IF: 4.996
    IL-37 alleviates Coxsackievirus B3-induced viral myocarditis via inhibiting NLRP3 inflammasome-mediated pyroptosis

    DOI: 10.1038/s41598-022-22617-y
    Sample: serum
  19. Annals of Translational Medicine (2023) IF: 3.616
    Recombinant human angiotensin-converting enzyme 2 plays a protective role in mice with sepsis-induced cardiac dysfunction through multiple signaling pathways dependent on converting angiotensin II to angiotensin 1–7

    DOI: 10.21037/atm-22-6016
    Sample: serum
  20. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    		 Sample: Heart
  21. Parasites & Vectors (2020) IF: 2.824
    Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model

    DOI: 10.1186/s13071-020-04104-3

    PMID: 32423469

    		 Sample: Serum
  22. Natural Product Communications (2022) IF: 1.496
    Phillyrin Inhibits Isoproterenol-Induced Cardiac Hypertrophy Via P38 and NF-κB Pathways

    DOI: 10.1177/1934578X221144581
    Sample: serum
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