NADH Oxidase (NOX) Activity Assay Kit (E-BC-K806-M)
For research use only.
| Detection Principle | NADH Oxidase（NOX）is widely found in the animals, plants, microorganisms and cultured cells, which can directly oxidize NADH to NAD+ in the presence of oxygen, and reduce blue DCPIP to colorless DCPIP. The activity of NOX can be calculated by measuring the reduction rate of blue DCPIP at 600 nm. |
| Synonyms | NOX |
| Sample Type | Plant,Animal tissue,Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-610 nm, optimum wavelength: 600 nm） |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.38 U/L |
| Detection Range | 0.38-22.09 U/L |
| Precision | inter-assay CV: 7.1% | intra-assay CV: 0.04 |
| Sample Volume | 20 μL(tissue/cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 5-10 |
| 10% Mouse heart tissue homogenate | 2-3 |
| 10% Porcine heart tissue homogenate | 1-3 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 3-5 |
| 10% Mouse muscle tissue homogenate | 1 |
| 10% Bovine liver tissue homogenate | 5-8 |
| 10%Epipremnum aureum tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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