PARN Polyclonal Antibody
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human
- Applications: WB;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:A549,MCF-7,HepG2,HeLa Verified Samples in IF: MCF-7 |
Dilution |
WB 1:500-1:2000, IF 1:50-1:100 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human PARN (NP_002573.1). |
Abbre | PARN |
Synonyms | PARN;DAN;DKCB6;PFBMFT4 |
Swissprot | |
Calculated MW | 52kDa/66kDa/67kDa/73kDa |
Observed MW |
73kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |